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A PROCESS FOR GENERATING CYTOPLASMIC MALE STERILE LINE IN RICE AND OTHER CROPS BY RNA EDITING
专利权人:
GRINENBERGER, JEAN-MICHEL;PATELL, VILLOO, MORAWALA;RAYAPURAM, NAGANAND;VENKATARAMIAH, MAHESH;JOY, JOMA;GOSWAMI, SULIP, KUMAR
发明人:
PATELL, VILLOO, MORAWALA,RAYAPURAM, NAGANAND,VENKATARAMIAH, MAHESH,JOY, JOMA,GOSWAMI, SULIP, KUMAR
申请号:
WO2001IN00024
公开号:
WO0162889(A2)
申请日:
2001.02.26
申请国别(地区):
世界知识产权组织国际局
年份:
2001
代理人:
摘要:
The present invention relates to a cytoplasmic male sterility (CMS) transgenic plant prepared by RNA editing for expressing unedited nad 9 gene disenabling ATP production in the mitochondria of plants and a process for preparing the same. The said process comprises: cloning an un-edited nad 9 gene by digesting the PCR product obtained from mitochondrial DNA to obtain <i>pNG3</i>cloning crop with un-edited nad 9 gene with the targeting sequence under ubiquitin promoter a NOS terminator to obtain <i>pNG11</i> co-bombarding <i>pNG11</i> constructs in a manner as herein described with hygromycin gene (pLAU6 hph construct) to generate plants containing un-edited nad 9 gene; analysing un-edited nad 9 (pNG11) construct with a control plant having edited nad 9 gene, <i>pNG10</i>to determine the presence of nad 9 gene.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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