Young Mee Jung;Sang-Heon Kim;Soo Hyun Kim;In Su Park
发明人:
Sang-Heon Kim,Soo Hyun Kim,In Su Park,Young Mee Jung
申请号：
US13259767
公开号：
US20120134965A1
申请日:
2010.03.24
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present invention relates to a method for differentiating stem cells into vascular cells by culturing them in the form of a three-dimensional cell cluster and the use of the three-dimensional cell cluster for angiogenesis. Specifically, the present invention teaches a method for differentiating stem cells into vascular cells comprising culturing stem cells by adhering them onto a culture plate with a surface having a hydrophobic property or a culture plate onto which a growth factor is immobilized, wherein the cultured stem cells are later detached from the culture plate as their density increases to form a three-dimensional cell cluster and grown in the form of a three-dimensional cell cluster while differentiating into vascular cells. Also disclosed is the use of a three-dimensional cell cluster composed of the vascular cells differentiated from stem cells by the above method as a cell therapy agent for angiogenesis.
