The invention provides a method for proofing effects of a seahorse extract, including: (a) a step of performing variety identification to a seahorse to identify the species of the seahorse, wherein the step includes: extracting a DNA sample from the seahorse and performing original identification on a gene of the DNA sample to identify the species of the seahorse and (b) a step of performing effect proof and/or quality determination to a seahorse extract extracted from the seahorse, wherein the step includes extracting the seahorse with a solvent to obtain the seahorse extract and performing a cancer cell viability test to the seahorse extract and/or determining whether the seahorse extract has at least one indicator compound.本發明提供一種海馬萃取物之功效驗證方法,包括:(a)將一海馬進行品種鑑定以確認該海馬之品種的步驟,其包括:萃取該海馬之一DNA樣本;以及將該DNA樣本一基因的一序列片段進行基原鑑定以確認該海馬之品種;以及(b)確認該海馬之品種後,將一萃取自該海馬之海馬萃取物進行功效確認及/或品質確認的步驟,其包括:以一溶劑來萃取該海馬以獲得該海馬萃取物;以及將該海馬萃取物進行癌細胞細胞存活率測試及/或確認該海馬萃取物是否包含至少一指標化合物。S1、S2‧‧‧步驟
