The present invention relates to a group of glycosyltransferases and their applications. Specifically, glycosyltransferases GT29-32, GT29-33, GT29-34, GT29-4, GT29-5, GT29-7, GT29-9, GT29-11, GT29-13, GT29-17, GT29-18 , GT29-19, GT29-20, GT29-21, GT29-22, GT29-23, GT29-24, GT29-25, GT29-36, GT29-37, GT29-42, GT29-43, GT29-45, GT29 -46, PNUGT29-1, PNUGT29-2, PNUGT29-3, PNUGT29-4, PNUGT29-5, PNUGT29-6, PNUGT29-7, PNUGT29-8, PNUGT29-9, PNUGT29-14 and PNUGT29-15 and their derived polypeptides By catalyzing the first glycosyl group of the C-20 moiety, the first glycosyl group of the C-6 moiety and the first glycosyl group of the C-3 moiety to extend the oligosaccharide, Ginsenoside Rg3, Ginsenoside Rd, Ginsenoside Rb1, Ginsenoside Rb3, Saponin DMGG, Saponin DMGX, Stevenleaf LXXV, Zipenoside XVII, Zipenoside XIII, Zippe Noside IX, notoginsenoside U, and notoginsenoside R1, Tojin ginsenoside R2, Noto ginsenoside R3, 3-O-β- (D-xylopyranosyl) -β- (D-glucopyranosyl) -PPD; A catalytic reaction was provided to obtain ginsenoside products such as 3-O-β- (D-xylopyranosyl) -β- (D-glucopyranosyl) -CK, 20-O-Glucosylginsenoside Rf and Ginsenoside F3. The glycosyltransferases of the present invention may also be applied to construct artificial synthetic ginsenosides and various novel ginsenosides and derivatives thereof.본 발명은 한 그룹의 글리코실트랜스퍼라제 및 이의 응용에 관한 것이다. 구체적으로, 글리코실트랜스퍼라제 GT29-32, GT29-33, GT29-34 , GT29-4, GT29-5, GT29-7, GT29-9, GT29-11, GT29-13, GT29-17, GT29-18, GT29-19, GT29-20, GT29-21, GT29-22, GT29-23, GT29-24, GT29-25, GT29-36, GT29-37, GT29-42, GT29-43, GT29-45, GT29-46, PNUGT29-1, PNUGT29-2, PNUGT29-3, PNUGT29-4, PNUGT29-5, PNUGT29-6, PNUGT29-7, PNUGT29-8, PNUGT29-9, PNUGT29-14와 PNUGT29-15 및 이의 유도 폴리펩티드가 테트라시클릭트리터페노이드계 화합물 기질 C-20 부위의 첫 번째 글리코실기, C-6 부위의 첫 번째 글리코실기 및 C-3 부위의 첫 번째 글리코실기에 촉매 작용을 하여 당사슬을 연장시킴으로써, 진세노사이드(ginsenoside)Rg3, 진세노사이드Rd, 진세노사이드Rb1, 진세노사이드Rb3, 사포닌(saponin)DMGG, 사포닌DMGX, 지페노사이드(Stevenleaf)LXXV, 지페노사이드XVII, 지페노사이드XIII, 지페노사이드IX, 노토진세노사이드(notoginseno