REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人:
WOO, HEE JONG,SHIN, KONG SIK,LIM, MYUNG HO,KWEON, SOON JONG,SUH, SEOK CHEOL,LEE, JIN HYOUNG
申请号：
KR20110119972
公开号：
KR20130054544(A)
申请日:
2011.11.17
申请国别(地区):
韩国
年份:
2013
代理人:
摘要：
PURPOSE: A plant transformation vector, pCMF, for removing a selective marker is provided to enable easy modification of the vector and transduction of a target gene, and to develop marker-free GM(genetically modified) crops. CONSTITUTION: A site-specific recombinase gene, mFLP, has a base in sequence number 1. A vector for transformation in a plant contains the gene, mFLP. The vector contains a stress-induced promoter and recombinase recognition site. The vector is able to remove a selective marker by induction of mFP expression and self-cleavage due to stress. The vector contains MCS(multi-cloning site). A plant is transformed with the vector. Agrobacterium tumefaciens LBA4404 is transformed by the vector and is used as a plant carrier. A transgenic plant is prepared by transforming with the vector and is able to remove the selective marker.
