A sampling and cutting device (14), the sampling and cutting device (14) is used for slicing and sampling a seed (5) arranged on a carrying surface (1). The seed (5) is tightly clamped by a clamping mechanism (11) and the carrying surface (1) in a cooperative manner and a portion of the seed (5) protrudes out of the carrying surface (1). The sampling and cutting device (14) comprises: a rotary cutting member (2) designed to cut the portion of the seed (5) protruding out of the carrying surface (1) at the lower portion of the seed (5) along the edge of the carrying surface (1), so as to form a cutting slit penetrating through the lower portion of the seed (5) a punching device (3) of which a punch (4) is positioned above the seed (5), a lower die consisting of the carrying surface (1), the punching device (3) being designed to punch, from top to bottom corresponding to the cutting slit, the portion of the seed (5) protruding out of the carrying surface (1) from the seed (5) having a slit cut by the rotary cutting device (2) a distance measuring device designed to measure the thickness of the seed (5) in the cutting plane of the rotary cutting member (2), the cutting depth of the seed (5) by the rotary cutting member (2) being controlled according to the thickness measured by the distance measuring device, so that the upper portion of the seed (5) remains connected with the seed after being cut by the rotary cutting member (2). An automatic seed slicing and sampling equipment comprising the sampling and cutting device (14), the sampling and cutting device (14) is used for cutting the seed (5) clamped onto the carrying surface (1) of a slicing and sampling position (10) by the clamping mechanism (11). The sampling and cutting device (14) can cut the seed (5) with high speed and is capable of cutting a portion having a substantially constant volume or mass from the seed (5).La présente invention concerne un dispositif déchantillonnage et de coupe (14), le dispositif déc