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Method for identifying t-cell stimulating protein fragments
专利权人:
Florian Kern
发明人:
Florian Kern,Hans-Dieter Volk,Peter Walden,Alexander Scheffold,Rainer Blasczyk
申请号:
US09600564
公开号:
US08932806B1
申请日:
1999.01.15
申请国别(地区):
US
年份:
2015
代理人:
摘要:
The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen b) subdividing the found amino acid sequence of the antigen into protein fragments c) synthesizing at least one protein fragment d) incubating a suspension containing t-cells with the protein fragments e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.
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