There is provided a method capable of manufacturing a mold at low cost, and a method of manufacturing a biocompatible pattern sheet by using the mold. The method of manufacturing a mold 68 having a recessed pattern 70 includes the steps of: forming a silicone resin film 60 by applying a silicone resin solution 58 to a surface of a model 40 having a protrusion pattern 42； defoaming the silicone resin film 60 by reducing the silicone resin film 60 in pressure； forming the mold 68 by heating and curing the silicone resin film 60 while a base plate 64 is in contact with a face of the silicone resin film 60, the face being opposite to the model 40； and releasing the mold 68 from the model 40 after releasing the base plate 64. The method of manufacturing a pattern sheet 76 uses the mold 68.
