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Transformation of algal cells
专利权人:
Bertrand Vick
发明人:
Bertrand Vick,Oliver Kilian
申请号:
US12480611
公开号:
US08119859B2
申请日:
2009.06.08
申请国别(地区):
US
年份:
2012
代理人:
摘要:
Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
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