A steam pasteurization method for freeze-dried food comprises: 1) determining a corresponding pasteurization temperature according to a type of a freeze-dried food item requiring pasteurization, and a target water activity at the pasteurization temperature; 2) cooling and lowering the temperature of the food item in step 1) to a cooling temperature corresponding to the food type; 3) removing air in a pasteurization chamber accommodating the food item by means of a vacuum pump; 4) charging steam into the pasteurization chamber, after the temperature of the food item reaches the pasteurization temperature, regulating a flow of the steam to keep the food item temperature at the pasteurization temperature for a specific duration, and then terminating the charging of the steam; 5) capturing the steam and lowering the pressure in the pasteurization chamber; and 6) after the pressure in the pasteurization chamber reaches or approximates a water saturation pressure corresponding to the cooling temperature before pasteurization, charging clean air into the pasteurization chamber until the chamber is not in a vacuum state, and discharging the food item to complete the process.