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Thiobenzoylation method of peptide sequencing with gas chromatography and mass spectrometric detection
专利权人:
CALIFORNIA INST OF TECHN
发明人:
STOLOWITZ MARK L
申请号:
AU19930046468D
公开号:
AU4646893(A)
申请日:
1993.06.17
申请国别(地区):
澳大利亚
年份:
1994
代理人:
摘要:
A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.
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