INTEGRATION MULTICOPIE ET EXPRESSION DE GENES HETEROLOGUES DANS UN METHYLBACTERIUM

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专利权人:

NATIONAL RESEARCH COUNCIL OF CANADA

发明人:

MIGUEZ, CARLOS B.,CHOI, YOUNG-JUN

申请号：

CA2553485

公开号：

CA2553485C

申请日:

2006.07.25

申请国别(地区):

CA

年份:

2014

代理人:

摘要：

The integration of genes into methylobacterium, such as M. extorquens ATCC 55366 is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (P mxaF). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for M. extorquens.

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