Federalnoe gosudarstvennoe byudzhetnoe uchrezhdenie nauki "Kirovskij nauchno-issledovatelskij institut gematologii i perelivaniya krovi Federalnogo mediko-biologicheskogo agentstva"
发明人:
Kostyaev Andrej Aleksandrovich (RU),Костяев Андрей Александрович (RU)
申请号：
RU2019101365
公开号：
RU0002711152C1
申请日:
2019.01.17
申请国别(地区):
RU
年份:
2020
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to technology of freezing peripheral blood precursor cells with Hemicolite cryopreservation. Freezing of peripheral blood precursor cells (PBPC) is carried out in stages: mixing it with concentrate of PBPC in equal proportions in a disposable plastic container, then sealing it, passportization, placing in metal pencil-holder, followed by stage-by-stage freezing of cell suspension from plus 4 °C to minus 80 °C in electric refrigerators with adjustable cold adaptation temperature of cells, defrosting in water bath to temperature of plus 2÷4 °C in the cell suspension, wherein at the first stage of freezing, the containers with the PBPC suspension are held for 10 minutes in an electric freezer, with a set temperature of plus 4 °C, at the second stage, the biological object is transferred into an electric freezer at a temperature of minus 28±1 °C is maintained in these conditions for 25 minutes, after which biological object is transferred into a freezer-storage with temperature minus 80±2 °C for final freezing and prolonged cryopreservation of PBPC.EFFECT: invention improves the technological level and effectiveness of hematopoietic stem cell cryopreservation by using modern industrial equipment and minimizing the risks of post-transfusion complications, accessible to medical institutions experiencing problems in providing liquid nitrogen, and safe for health of technical personnel.1 cl, 1 tbl, 1 exИзобретение относится к медицине, а именно к технологии замораживания клеток-предшественников периферической крови с криоконсервантом Гекмолит. Замораживание клеток-предшественников периферической крови (КППК) проводят поэтапно: производят смешивание его с концентратом КППК в равных пропорциях в одноразовом пластиковом контейнере, затем его герметизируют, паспортизируют, помещают в металлический пенал-холдер, после чего осуществляют поэтапное замораживание клеточной взвеси от плюс 4°С до минус 80°С в электрически
