Jens Holger Vogel;Roberto Giovannini;Paul Wu;Huong My Nguyen;Konstantin B. Konstantinov
发明人:
Jens Holger Vogel,Roberto Giovannini,Konstantin B. Konstantinov,Huong My Nguyen,Paul Wu
申请号：
US11576097
公开号：
US09045725B2
申请日:
2005.09.30
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system； and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.
