The instant disclosure provides a disinfection method which can perform exactly and highly ultraviolet disinfection even though the bacteria have low ultraviolet light transmittance (e.g., the bacteria of food or beverage), such that the purification process of the material having the ultraviolet light transmittance in the ultraviolet light irradiation device can be substantially omitted. Therefore, the risk of recontamination can be avoided.The ultraviolet light disinfection method of the instant disclosure is that, after the bacteria are filled into the ultraviolet light transmittable container which is a bag (e.g., a flexible bag) made from a ultraviolet light transmittable film composed of heat-sealing resin, the ultraviolet light is used to irradiate outside of the ultraviolet light transmittable container for killing the bacteria therein, the ultraviolet light transmittable container containing the bacteria is hermetically sealed during the time from after filling the bacteria to completing the ultraviolet light irradiation under a sterile environment.本發明提供一種殺菌方法,即便對於例如像食品或飲料等那樣紫外線透過率低之被殺菌體亦能夠進行確實性高之紫外線殺菌,可實質地省略紫外線照射裝置中之紫外線透過性窗口材料之淨化作業,進而再次污染之危險性小。本發明之紫外線殺菌方法於向例如紫外線透過性容器為將由熱密封性樹脂所構成之紫外線透過性膜製袋而成的可撓性袋等該種的紫外線透過性容器中填充被殺菌體後,自前述紫外線透過性容器之外部照射紫外線而進行被殺菌體之殺菌時,於前述填充結束後至紫外線照射結束前之期間內將填充有被殺菌體之容器加以氣密地密封,或於同一無菌環境下進行紫外線照射及前述密封。100‧‧‧紫外線殺菌裝置110‧‧‧紫外線發光模組115‧‧‧深紫外線發光元件之光軸120‧‧‧包含長橢圓反射鏡之出射側反射鏡130‧‧‧聚光紫外線出射單元200‧‧‧被殺菌體300‧‧‧紫外線透過性容器
