The present invention provides Bacillus subtilisisolated strains, a method for preparing the fermentation extract of the strains, the fermentation extract prepared thereby, a composition comprising the fermentation extract, and uses of the fermentation extract for promoting chondrocyte regeneration, increasing cartilage extracellular matrix secretion and/or treating arthritis.本發明提供枯草芽孢桿菌( Bacillus subtilis)分離株、製備其發酵萃取物之方法、其發酵萃取物、包含其發酵萃取物之組成物,及其發酵萃取物用於促進軟骨細胞再生、增加軟骨細胞外間質分泌及/或治療關節炎之用途。