DOUBLE-FUNCTIONAL OLIGONUCLEOTIDE COMPRISING COMPLEMENTARY NUCLEOTIDE SEQUENCE, MIS-MATCHED NUCLEOTIDE SEQUENCE, REPORTER, AND QUENCHER, AND METHODS FOR NUCLEIC ACID AMPLIFICATION AND MEASUREMENT USING SAME
WON, Yoo Deok,PARK, Hae Joon,SEONG, Hyo Jin,LEE, Sun Young
申请号：
WO2016KR05517
公开号：
WO2016190654(A1)
申请日:
2016.05.25
申请国别(地区):
世界知识产权组织国际局
年份:
2016
代理人:
摘要：
The present invention relates to a complementary double-stranded oligo, wherein, for the amplification of a particular gene sequence, an inosine linker is linked to the 5'-terminus of a primer for the corresponding sequence, a sequence complementary to the primer is linked to the inosine linker, and at least one mis-matched nucleotide is included in the complementary sequence to form a bubble structure; wherein, depending on the treatment temperature, at a predetermined temperature or lower, a single stranded oligo is turned into a double-stranded form to exist in an inactivation form, and at a predetermined temperature or higher, the oligo is activated into a single-stranded oligo; and wherein, a fluorescent substance (reporter dye) and a quenching material (quencher molecule) are attached to the oligo, so that the oligo can be applied as a primer or a probe, and thus only two oligos can realize the gene amplification and fluorescent signal real-time measurement with high specificity, and to a measuring meth
