A sperm quality assessment device includes a main frame, and a test pad. The test pad is encircled by the main frame and includes an exposed introductory portion. The test pad has greater hydrophilicity than the main frame and has an MTT reagent distributed therein. Therefore, activity pattern of the dyed motile sperms can be utilized to estimate sperm quality such as mobility. The present device in particular has the merits of adopting paper material to reduce manufacture cost and is also easy to use.本發明揭露一種精子品質檢測裝置,包括一主體與一測試墊,其中測試墊被主體包圍,並包含有裸露之一導入部。測試墊的親水性大於主體的親水性,且測試墊中散佈有一MTT染劑。藉此,被染色之活性精子在測試墊上的活動分布可作為評估精子品質如活性程度之用。特別是檢測裝置可使用紙材來達到製作成本低廉以及使用容易之優點。