CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.)
发明人:
LOUIS CASTEILLA,VALERIE PLANAT BENARD,LUC PENICAUD,CARINE CHANUT
申请号：
FR0600710
公开号：
FR2896511B1
申请日:
2006.01.26
申请国别(地区):
FR
年份:
2012
代理人:
摘要：
Process to obtain cardiac cells, comprises selecting cardiomyogenic cells from raw stromal vascular fraction (SVF), culturing the cells in an optimized liquid medium for the ex vivo expansion of the cells, maintaining and expanding the cells by successive passage in liquid medium and obtaining the cardiac cells. An independent claim is included for a composition comprising: adherent contractile cardiomyogenic cells in elongated form such as CD31 ->, CD38 +>, CD44 +>, CD45 ->, CD73 +>, CD81 +>, MLC2v +>, Ckit ->, CMH2 ->, where approximately 50% of the cells being Sca-1 +> and troponin T +> and non-adherent cells in round form such as CD31 ->, CD38 ->, CD44 +>, CD45 ->, CD73 ->, CD81 +>, CMH1 ->, where approximately 50% of the cells being Sca-1 +>, troponin T +> and MLC2v +>, two types of cells further expressing the mesodermic factor of transcription Brachury, the transcription factors islet-1 and MEF-2c and the binding transcriptional factor Oct3/4 and an excipient. ACTIVITY : Cardiant Vasotropic. The ability of the cardiac cell to reconstitute ischemic cardiac zone was tested in mouse using biological assays. The results showed that after 7 days of treatment the ischemic zone was regenerated, which was analyzed using green fluorescent protein specific marker. MECHANISM OF ACTION : None given.Procédé de culture de cellules issues du tissu adipeux et notamment de la fraction stroma-vasculaire (FSV ou SVF pour stromal vascular fraction) pour induire la formation de cardiomyocytes, utilisation des cellules obtenues par ledit procédé de culture pour reconstituer une zone cardiaque ischémiée, notamment après infarctus, ainsi que composition pharmaceutique contenant lesdites cellules.Ledit procédé dobtention de cellules cardiaques comprend au moins les étapes suivantes :a) sélection des cellules cardiomyogéniques à partir de la fraction stroma-vasculaire (FSV ou SVF pour stromal vascular fraction) brute b) culture des cellules sélectionnées à létape a) dans un milieu liq
