STICE STEVEN L.,CIBELLI JOSE,ROBL JAMES,GOLUEKE PAUL,PONCE DE LEON F. ABEL,JERRY D. JOSEPH
申请号：
US19970935052
公开号：
US6235970(B1)
申请日:
1997.09.22
申请国别(地区):
美国
年份:
2001
代理人:
摘要：
An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
