A docking station for maintaining sterilization of temporarily disconnected male medical line connectors. The docking station has a plurality of docking bays, each of which retains an ampule therein containing sterilization fluid and has an access mouth exteriorly exposed when the ampule is secured in its respective bay. The mouth of the ampule is provided with a female medical line connection for temporarily receiving and securing one of the male line connector temporarily disconnected from a patient. A tubular tip and the concentric connection collar of the one of the male medical line connectors is thereby immersed in the sterilization fluid for sterilization while docked.