The subject of the invention is a microprobe for selective electroporation comprising at least two metal electrodes (A) immersed in a glass rod (E), characterised in thatthe glass rod (E) made of a primary glass is 50 µm to 2 mm in diameter, preferably 50 µm to 500 µm,the metal electrodes (A) made of a metal alloy are formed as rods with diameter of 1 µm to 100 µm, preferably 20 µm to 30 µm, wherein endings of those rods are exposed,wherein the primary glass and the metal alloy are matched in such manner that dilatometric softening temperature DTM of the primary glass is highly similar to the temperature of melting for the metal alloy.The invention also includes a method of manufacturing of such a microprobe.