Thiobenzoylation method of peptide sequencing with gas chromatography and mass spectrometric detection

• 收藏
• 分享

专利权人:

CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人:

STOLOWITZ, MARK L.

申请号：

US19920919524

公开号：

US5246865(A)

申请日:

1992.07.24

申请国别(地区):

美国

年份:

1993

代理人:

摘要：

A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/