SHIVA PRASAD KOTHA;ANDRE RONALD WATSON;RENSSELAER POLYTECHNIC INSTITUTE;VAIBHAV A. PANDIT
发明人:
SHIVA PRASAD KOTHA,ANDRE RONALD WATSON,VAIBHAV A. PANDIT
申请号：
IL24458516
公开号：
IL244585A
申请日:
2016.03.14
申请国别(地区):
IL
年份:
2019
代理人:
摘要：
An improved nanoparticle for transfecting cells is provided. The nanoparticle includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. Also provided is an improved method of modifying intracellular polynucleotides. The method includes contacting a cell with a nanoparticle that includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.
