AMGEN RESEARCH (MUNICH) GMBH;MICROMET AG;MICROMET GMBH;MICROMET GESELLSCHAFT FUER BIOMEDIZINISCHE FORSCHUNG MBH
发明人:
PETER KUFER,TOBIAS RAUM
申请号：
BR9809391
公开号：
BRPI9809391B1
申请日:
1998.04.14
申请国别(地区):
BR
年份:
2016
代理人:
摘要：
"Process for the production and use of anti-human antigen receptors". A process for producing an anti-human antigen receptor that is low immunogenic or non-immunogenic in humans is described, comprising the steps of selecting a combination of vh and vl immunoglobulin chains, wherein at least said vh chain is derived from essentially uninitiated mature human b lymphocytes or essentially anergic human b cells and said v1 chain is derived from a repertoire of natural human b cells, said chains being expressed from a recombinant vector and using a 'in vitro' exposure system for binding to a human antigen. furthermore, receptors that are low in immunogenicity or non-immunogenic in humans and directed to human antigens are provided, said receptors being obtainable by the process of the invention. said receptors are preferably antibodies or fragments thereof or immunoconjugates comprising the vh / vl chains of said antibody. In particular, the receptors are described directed to human tumor antigens, preferably to human tumor antigen 17-1a, also known as epcam, egp or ga 733-2. finally, kits useful for conducting the process of the invention and pharmaceutical compositions comprising the receptors mentioned above are provided.patente de invenção: <；b>；"processo para a produção de receptores de antígenos anti-humanos e usos dos mesmos"<；d>；. descreve-se um processo para produção de um receptor de antígeno anti-humano que é de baixa imunogenicidade ou não-imunogênico em seres humanos, compreendendo as etapas de seleção de uma combinação de cadeias de imunoglobulina vh e vl, em que pelo menos a dita cadeia vh é derivada de linfócitos b humanos maduros essencialmente não-iniciados ou de células b humanas essencialmente anérgicas e a dita cadeia vl é derivada de um repertório de células b humanas naturais, as ditas cadeias sendo expressadas a partir de um vetor recombinante e usando um sistema de exposição 'in vitro', para ligação em um antígeno de ser humano. além do m
