In the present invention, the royal jelly is eluted with water to obtain a residual, the residual obtained is eluted with 50% ethanol to obtain a residual, then the obtained residual is eluted with 99.5% ethanol And obtaining elution components for each of the fractions, thereby obtaining a royal jelly fraction.本発明は、ローヤルゼリーを水で溶出し残差を得ること、得られた残差を50%エタノールで溶出し残差を得ること、次いで、得られた残差を99.5%エタノールで溶出し溶出各分を得ることを含む、ローヤルゼリー分画の製造方法に関する。