Method, for separating and using full saliva hirudo extract. The method may include to phagostimulatory agent to leech Induction reflux leech, induction includes the temperature in leech environment below about 0 °C With collect unpurified, the cooling leech of full saliva reflux. The method may include readjust leech by making its heating, in temperature from about 5 °C to about 40 °C. Stable, freeze-drying, full saliva hirudo extract and the active stabilization of the extract, when being stored for being below about -20 °C in temperature, extract is kept at least 70% active at least six moon. The extract can be used for treating solid tumor, treatment liquid tumour, and treatment diabetes, treatment viral disease treat parasitic disease, treat antibacterial disease, or as antioxidant.
