您的位置: 首页 > 农业专利 > 详情页

非蛍光性蛍光蛋白質を用いた遺伝毒性を判定するための方法
专利权人:
发明人:
申请号:
JP20120556031
公开号:
JP5634531(B2)
申请日:
2011.03.31
申请国别(地区):
日本
年份:
2014
代理人:
摘要:

The present invention relates to a method for determining a genotoxicity of a test substance, comprising the steps of: (a) transforming a fish with a nucleotide sequence encoding a non-fluorescent fluorescence protein with a mutation; (b) treating a test substance to the transformed fish; and (c) measuring a fluorescence in the test substance-treated fish, wherein the fluorescence is generated by reversion of the non-fluorescent fluorescence protein to the fluorescence protein due to a back mutation of the nucleotide sequence in the test substance-treated fish. According to the present invention, MutaFish system, Zebrafish ( Brachydanio rerio ) line, in which the fluorescence protein variant, preferably wild type EGFP variant (EGFP mut ) is transformed, provides a much excellent animal system for measuring a genotoxicity of a test substance via production of a fluorescent fry larvae generated through reversion of the fluorescent protein variant caused by treatment of the test substance. Accordingly, the Mu

来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

意 见 箱

匿名:登录

个人用户登录

找回密码

第三方账号登录

忘记密码

个人用户注册

必须为有效邮箱
6~16位数字与字母组合
6~16位数字与字母组合
请输入正确的手机号码

信息补充