非蛍光性蛍光蛋白質を用いた遺伝毒性を判定するための方法
- 专利权人:
- 发明人:
- 申请号：
- JP20120556031
- 公开号：
- JP5634531(B2)
- 申请日:
- 2011.03.31
- 申请国别(地区):
- 日本
- 年份:
- 2014
- 代理人:
- 摘要：
The present invention relates to a method for determining a genotoxicity of a test substance, comprising the steps of: (a) transforming a fish with a nucleotide sequence encoding a non-fluorescent fluorescence protein with a mutation; (b) treating a test substance to the transformed fish; and (c) measuring a fluorescence in the test substance-treated fish, wherein the fluorescence is generated by reversion of the non-fluorescent fluorescence protein to the fluorescence protein due to a back mutation of the nucleotide sequence in the test substance-treated fish. According to the present invention, MutaFish system, Zebrafish ( Brachydanio rerio ) line, in which the fluorescence protein variant, preferably wild type EGFP variant (EGFP mut ) is transformed, provides a much excellent animal system for measuring a genotoxicity of a test substance via production of a fluorescent fry larvae generated through reversion of the fluorescent protein variant caused by treatment of the test substance. Accordingly, the Mu
- 来源网站：
- 中国工程科技知识中心
