The optical device has a holding device for holding a uniform carrier with an arrangement of multiple individual detection locations. A light source is provided for emitting light with an excitation frequency. An optical sensor is so arranged that it receives fluorescence signals of the arrangement of multiple individual detection locations and produces machine-readable primary data. An array optical arrangement (18) is provided which includes field lens array (19) with field lens array elements (20) and a pupil lens array (21) with pupil lens array elements (22). Independent claims are included for the following: (1) a real-time polymerase chain reaction device with a unit for heating and cooling a carrier; (2) an analytic system for simultaneous execution and monitoring of multiple polymerase chain reactions in real-time; and (3) a method for amplifying, detecting or quantifying multiple deoxyribonucleic acid-target sequence.
