Methods for constructing and producing a recombinant adenovirus based vector vaccine containing multiple influenza antigen genes for use in generating broad based immune responses against influenza A and B viruses and that allows for multiple vaccinations in individuals with preexisting immunity to adenovirus are described.本發明闡述用於構築及產生基於重組腺病毒之載體疫苗之方法,該載體疫苗含有多個流感抗原基因用於產生針對A及B型流感病毒之廣譜免疫反應且允許在對腺病毒具有既存免疫性之個體中進行多次疫苗接種。