PROBLEM TO BE SOLVED: To provide a treatment method and a treatment apparatus, having a high bactericidal action or enzyme deactivating action.SOLUTION: The method for applying treatment of at least either sterilization of a microorganism or deactivation of the enzyme to a liquid object to be treated includes: a bubble mixing step of mixing microbubbles of carbon dioxide at a first temperature T1 and under a first pressure P1 to the object to be treated； and a treatment step of holding the object to be treated at a second temperature T2 having higher temperature than that of the first temperature T1 and under a second pressure P2 having higher pressure than that of the first pressure P1.【課題】殺菌作用あるいは酵素失活作用が高い処理方法および処理装置を提供すること。【解決手段】微生物の殺菌と酵素の失活との少なくともいずれかの処理を液状の被処理物に対して行う処理方法であって、第一温度Ｔ１且つ第一圧力Ｐ１のもとで二酸化炭素の微細気泡を被処理物と混合する気泡混合工程と、第一温度Ｔ１より高い第二温度Ｔ２且つ第一圧力Ｐ１よりも高い第二圧力Ｐ２のもとで被処理物を保持する処理工程と、を備える。【選択図】図１
