The problem of contamination of sterile splash basins and surgical instrumentation used in operating rooms is solved by the use of an aqueous composition containing chlorhexidine gluconate in a concentration of sufficient strength to provide inhibition of bacterial growth and disinfection of surgical instruments, but is not harmful to non-mucosal tissues. Pre-filled bottles of the aqueous composition are also provided, with drip-free mouths that minimize contact with the exterior of the bottle. Also disclosed are pre-filled splash basins with removable covers that help maintain sterility. Also disclosed are pre-filled ampoules in sterile wrappers for dilution into sterile splash basins with dispensed sterile water.