Provided is a nutritional composition capable of promoting the secretion of GLP-1, after the finding that a nutritional composition including fermented milk, dietary fiber, and oligosaccharide promotes the secretion of GLP-1 which is an incretin hormone. This nutritional composition is useful for energy supplementation of subjects who have reduced glucose tolerance, such as diabetic patients, patients in highly invasive periods, and the elderly.本發明發現含有發酵乳、食物纖維及寡糖的營養組成物,促進腸泌素荷爾蒙之GLP-1分泌,本發明係提供可促進GLP-1分泌之營養組成物。本發明之營養組成物係可適用於糖尿病患者、高侵襲性醫療處置期患者、高齡者等糖耐受性低對象之能量補給。