The present application provides multispecific, such as trispecific, antigen binding proteins comprising a first antigen binding domain comprising a heavy chain variable domain and a light chain variable domain, a second antigen binding domain comprising a first single-domain antibody, and a third antigen binding domain comprising a second single-domain antibody. Pharmaceutical compositions comprising the multispecific antigen binding proteins, kits and methods of use thereof are further provided.本申請案提供多特異性諸如三特異性抗原結合蛋白，其包含：含有重鏈可變域及輕鏈可變域之第一抗原結合域、含有第一單域抗體之第二抗原結合域、及含有第二單域抗體之第三抗原結合域。進一步提供包含該等多特異性抗原結合蛋白之醫藥組合物、套組及其使用方法。
