An image segmentation threshold value determining method, comprising: defining a plurality of image regions of a first sensing image; determining a first, a second part image segmentation threshold values according to a first, second image regions of the image regions; performing a first, a second image segmentation operation to the first sensing image according to the first, the second part image segmentation threshold values to acquire a first, a second segmented images; and selecting one of the first part image segmentation threshold value and the second part image segmentation threshold value as the first image segmentation threshold value according to the first segmented image and the second segmented image.