A method for purifying and renaturating inclusion bodies of scorpion venom protein is provided. The method includes expressing the scorpion venom protein by recombinant Escherichia coli. The C-terminal of the scorpion venom protein has His-tag. The method includes breaking the disulfide bonds in the scorpion venom protein by a denaturating buffer, purifying the denaturated scorpion venom protein with a histidine affinity chromatography column, and renaturating the scorpion venom protein with a renaturation buffer. The renaturation buffer has a pH of 7-9 and includes 50-200 mmol/L Na2HPO4, 10-100 mmol/L Tris, 0.1-1 mol/L L-Arg, 1-5 mmol/L EDTA, 0.1-5 mmol/L GSH, 0.05-0.5 mmol/L GSSG, 5-20% (v/v) glycerol, 0.01-5% (v/v) triton X-100. Preparation of the scorpion venom protein by this method has the advantages of simple operation, and good renaturation effect.
