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TECHNOLOGY OF PRODUCTION OF MACROMOLECULAR MICROSPHERES
专利权人:
НексБио Инк. (US)
发明人:
ФАНГ Фанг (US),МАЛАХОВ Майкл П. (US)
申请号:
RU2012131203/15
公开号:
RU2012131203A
申请日:
2012.07.11
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A method of manufacturing a protein-based composition, comprising: a) adding a counterion to a solution containing protein in an aqueous solvent; b) adding an organic solvent to this solution; iv) gradual cooling of the solution to a temperature below about 25 ° C, resulting in the formation of a composition containing microparticles containing protein, where stages (a), (b) and (c) are carried out simultaneously, sequentially, intermittently or in any order. 2. The method according to claim 1, where stages (a) and (b) are carried out simultaneously or sequentially in any order, and then stage (c) is performed. The method according to claim 1, where stages (a), (b) and (c) are carried out sequentially in the following order: (a), then (b), then (c). 4. The method of claim 1, wherein the organic solvent is mixed or partially mixed with an aqueous solvent. The method according to claim 1, further comprising after step (c) separating the microparticles from the solution in order to remove components that are not microparticles. The method according to claim 1, wherein the organic solvent is selected from aliphatic alcohols, aromatic alcohols, chloroform, dimethyl chloride, polyhydric sugar alcohols, aromatic hydrocarbons, aldehydes, ketones, esters, ethers, dioxanes, alkanes, alkenes, conjugated dienes, dichloromethane, acetonitrile , ethyl acetate, polyols, polyimides, polymers of esters, polyaldehydes and mixtures thereof. 7. The method of claim 6, wherein the organic solvent is an aliphatic alcohol or aromatic alcohol. The method of claim 7, wherein the organic solvent is an aliphatic alcohol. The method of claim 8, wherein the aliphatic alcohol is isopropanol. C1. Способ изготовления композиции на основе белка, включающий:а) добавление противоиона к раствору, содержащему белок в водном растворителе;б) добавление органического растворителя к данному раствору; ив) постепенное охлаждение раствора до температуры ниже примерно 25°С, в результате чего образуется компо
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