PURPOSE: A method for determining tomato spotted wilt virus resistance using tomato spotted wilt virus is provided to evaluate resistance of improved Capsicum annuum and tomato species. CONSTITUTION: A method for determining tomato spotted wilt virus resistance in Capsicum annuum species comprises: a step of isolating RNA from a target Capsicum annuum species and preparing cDNA; a step of performing PCR using a PCR primer set corresponding to DNA encoding one nucleocapsid protein among sequence numbers 1-5; and a step of determining the presence of sequences corresponding to the sequence numbers 1-5.