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METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS
专利权人:
General Electric Company
发明人:
Kvam Erik Leeming,Nelson John Richard,GROSSMANN Gregory Andrew,Heller Ryan Charles,Finehout Erin Jean,Puleo Christopher Michael,Waters William Patrick
申请号:
US201715463943
公开号:
US2017211059(A1)
申请日:
2017.03.20
申请国别(地区):
美国
年份:
2017
代理人:
摘要:
Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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