PROBLEM TO BE SOLVED: To provide a herbicidal composition efficiently and effectively used for extermination of an adverse plant, and to provide an extermination method of an adverse plant.SOLUTION: A herbicidal composition comprising an enzyme is used for extermination of an adverse plant and has hydrolysis activity of p-Nitrophenyl butyrate. The composition comprises: a cutinase-like enzyme having no pathogenicity against a plant and derived from a microorganism and a plant pathogenic microorganism being a herbicidal pesticide. The content of the cutinase-like enzyme in the herbicidal composition comprising the enzyme is 0.01 U/mL or more in terms of the hydrolysis activity of p-Nitrophenyl butyrate.COPYRIGHT: (C)2014,JPO&INPIT【課題】効率的且つ効果的な有害植物の駆除に使用される除草用の組成物、及び有害植物の駆除方法を提供すること。【解決手段】有害植物を駆除するための除草用酵素含有組成物であって、酪酸p-ニトロフェニル加水分解活性を有し、植物に対して病原性を有さない微生物由来のクチナーゼ様酵素と、除草用農薬である植物病原性微生物と、を含み、除草用酵素含有組成物における前記クチナーゼ様酵素の含有量が、酪酸p-ニトロフェニル加水分解活性に換算して0.01U/mL以上である除草用酵素含有組成物。【選択図】図6