Multi-domain recombinant proteins having a cutinase catalytic domain are provided. The cutinase catalytic domain is a modular domain, which may be combined with other protein domains that also function in a modular fashion. For example, the cutinase catalytic domain can be operably linked to a polymer binding domain via a linker domain (e.g., a threonine/proline-rich linker polypeptide). In some embodiments, the cutinase catalytic domain is from an endogenous cutinase having a multi-domain modular organization, such as from the actinobacterium Kineococcus radiotolerans. Compositions, methods and uses of the recombinant proteins are also disclosed, such as for use in industrial applications.
