PROBLEM TO BE SOLVED: To provide a method for separating a desired antibody from a mixture of compounds given to cells and from a byproduct of the cells themselves with sufficient purity for use as a human therapeutic.SOLUTION: The present invention provides a method for purifying an antibody from a composition comprising the antibody and at least one contaminant. The method comprises the sequential steps of: (a) loading the composition onto a cation exchange material, the composition being at a first pH； (b) washing the cation exchange material with a first wash buffer at a pH greater than that of the composition in (a), the pH of the first wash buffer being from about 6.8 to about 9.0； (c) washing the cation exchange material with a second wash buffer at a pH which is less than that of the first wash buffer； and (d) eluting the antibody from the cation exchange material with an elution buffer at a conductivity substantially greater than that of the second wash buffer.SELECTED DRAWING: None【課題】細胞に与えた化合物の混合物から、また細胞自体の副生成物から所望の抗体を、ヒトの治療用として使うために十分な純度で分離する方法の提供。【解決手段】前記抗体と少なくとも一の汚染物質を含有する組成物から前記抗体を精製する方法であって、(ａ)陽イオン交換材料に前記組成物をロードし、ここで前記組成物は第１のｐＨにあり；(ｂ)(ａ)の前記組成物のｐＨよりも高いｐＨで第一洗浄バッファーを用いて前記陽イオン交換材料を洗浄し、ここで前記第一洗浄バッファーのｐＨは約６．８～約９．０であり；(ｃ)前記第一洗浄バッファーのｐＨより低いｐＨで第二洗浄バッファーを用いて前記陽イオン交換材料を洗浄し；(ｄ)前記第二洗浄バッファーよりも実質的に高い伝導率で溶出バッファーを用いて前記陽イオン交換材料から抗体を溶出させる逐次工程を含む方法。【選択図】なし
