ЛЮ Хой Ф. (US),КЕЛЛИ Брайан Дэвид (US),МАЙЕРС Дианна Е. (US),МАККУИ Бет (US),ПЕТТИ Криста Мари (US)
申请号：
RU2012156271/04
公开号：
RU2012156271A
申请日:
2011.05.25
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method of purifying a polypeptide from a composition comprising a polypeptide and at least one contaminant, wherein the method comprises (i) or (ii) :( i) sequential steps (a) of loading a cation exchange material with a composition with a loading density of more than 150 g / l cation exchange material； and (b) loading the mixed-type material with a composition recovered from the cation exchange material； or (ii) successive steps of (a) loading the mixed type of material with the composition； and (b) loading the cation exchange material with a composition extracted from a mixed type material with a loading density of more than 150 g / l of cation exchange material. The method according to claim 1, in which the polypeptide is characterized by a pH value of approximately from 6 to 10.3. The method according to claim 2, in which the polypeptide is characterized by a pH value of approximately from 7 to 9.4. The method according to any one of claims 1 to 3, in which the polypeptide is an antibody or immunoadhesin. The method of claim 4, wherein the polypeptide is immunoadhesin. The method of claim 4, wherein the polypeptide is an antibody. The method of claim 6, wherein the antibody is a monoclonal antibody. The method of claim 7, wherein the monoclonal antibody is a chimeric antibody, a humanized antibody, or a human antibody. The method of claim 7, wherein the monoclonal antibody is an IgG monoclonal antibody. The method of claim 6, wherein the antibody is an antigen binding fragment. The method of claim 10, wherein the antigen binding fragment is selected from the group consisting of a Fab fragment, Fab'fragment, F (ab ') fragment, scFv, Fv, and diabody. The method according to claim 1, where at least one zag1. Способ очистки полипептида из композиции, содержащей полипептид и по меньшей мере одно загрязняющее вещество, где способ включает (i) или (ii):(i) последовательные стадии (а) загрузки катионообменного материала композицией с плотностью загрузки более чем 1
