A process for anti-calcification of a tissue is disclosed. The process comprises treating a tissue with a pre-heated solution of fixative agent, treating the tissue with a sterilant, masking the tissue with a solution of an alkylamine in phosphate buffer saline to yield a masked tissue, reducing the masked tissue with a solution of lithium aluminum hydride thereby yielding a reduced tissue and washing the reduced tissue with an alcohol to yield an anti-calcified tissue. IN201721040401A