Methods and systems for supporting remote unit uplink tests in a distributed antenna system (DAS) are disclosed. In this regard, in one aspect, a centralized test signal generator is provided in the DAS to generate uplink test signals for one or more remote antenna units (RAUs) under test (RUTs). By providing the uplink test signals to the one or more RUTs from the centralized test signal generator, it is possible to perform uplink tests more efficiently with reduced equipment cost. The uplink test signals are distributed to the one or more RUTs over a downlink communications medium of the DAS. In another aspect, the one or more RUTs return the uplink test signals to a signal monitor over an uplink communications medium of the DAS, whereby uplink performance of the one or more RUTs can be examined to help optimize the DAS to improve quality of service (QoS).