イネ属の植物を形質転換する方法
- 专利权人:
- 独立行政法人農業・食品産業技術総合研究機構
- 发明人:
- 小沢 憲二郎
- 申请号：
- JP2008212554
- 公开号：
- JP5334098B2
- 申请日:
- 2008.08.21
- 申请国别(地区):
- JP
- 年份:
- 2013
- 代理人:
- 摘要：
PROBLEM TO BE SOLVED: To provide a method for transforming a plant of the genus Oryza by an Agrobacterium method, which is a universal method applicable to a large number of rice plant varieties including useful varieties and has a remarkably high transformation efficiency, and a method for obtaining a plant body of a transformed plant of the genus Oryza without carrying out a selection using a selection marker gene.
SOLUTION: The method for transforming the plant of the genus Oryza by an Agrobacterium method includes: immersing a callus of plant of the genus Oryza in a suspension obtained by culturing and suspending Agrobacterium to which a foreign gene is previously transferred then standing the callus on filter paper having absorbed a liquid medium for cocultivation in an amount of 90-110% of a liquid to be absorbed and carrying out cocultivation with Agrobacterium so as to introduce the foreign gene into the callus in high efficiency.
COPYRIGHT: (C)2010,JPO&INPIT
- 来源网站：
- 中国工程科技知识中心
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