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AGROBACTERIUM-MEDIATED METHOD FOR PRODUCING TRANSFORMED PLANT
专利权人:
JAPAN TOBACCO INC.
发明人:
Yuji ISHIDA,Yukoh HIEI
申请号:
US13717663
公开号:
US20130125265A1
申请日:
2012.12.17
申请国别(地区):
US
年份:
2013
代理人:
摘要:
The present invention aims to provide a novel Agrobacterium-mediated method for producing a transformed plant.The method of the present invention is an Agrobacterium-mediated method for producing a transformed plant, which comprises:(i) a coculture step for culturing an Agrobacterium-inoculated plant material with a coculture medium and(ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium, either without callus growth culture or after callus growth culture, to thereby regenerate a whole plant,wherein 1) said method comprises transformation enhancement, andwherein 2) said method does not comprise selection of transformed cells based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.
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