A method for manufacturing a pixel structure is provided. A patterned semiconductor material layer, an insulation material layer, and a gate electrode material layer are formed in sequence on a substrate to form a stacked structure. A patterned photoresist layer is formed on the stacked structure by using a photomask. A portion of the stacked structure is removed to pattern the patterned semiconductor material layer into a patterned semiconductor layer by using the patterned photoresist layer as a mask. Another portion of the stacked structure is etched by using a portion of the patterned photoresist layer as a mask until a portion of the semiconductor layer in the stacked structure is exposed. Then, an exposed portion of the semiconductor layer is modified to increase a conductivity of the exposed portion of the semiconductor layer. Finally, the patterned photoresist layer is removed. A pixel structure manufactured by the method is provided.