The present invention relates to a kit for preparing double-function and double-action targeted nano-immuno liposome, the liposome is mainly obtained by combining radio-drug and chemical therapy drug, the kit comprises (1) vial A: protein drug (2) vial B: Traut’s (2-imonothiolane-HCl) reagent (3) vial C: reagents including DSPC, cholesterol, mPEG-DSPE, DSPE-PRG-maleimide and chemical therapy drug (4) vial D: reagents including BMEDA, gluconate acetate and SnCl2 and (5) vial E: radio isotope solution. The present invention also relates to a method for preparing double-function and double-action targeted nano-immuno liposome by using the kit.本發明有關一種製造雙功能與雙效奈米靶向性免疫微脂體之套組,其主要是由結合放射藥物與化學治療藥物而成,該套組包含:(1)A瓶:蛋白質藥物;(2)B瓶:Traut’s(2-亞胺基四氫噻吩-HCl)試劑:(3)C瓶:包含DSPC、膽固醇、mPEG-DSPE、DSPE-PEG-馬來西亞胺及化療藥物;(4)D瓶:包含BMEDA、葡糖酸酯乙酸酯及SnCl2;及(5)E瓶:放射線同位素溶液。本發明亦有關使用該套組製造雙功能與雙效奈米靶向性免疫微脂體之方法。