NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY
发明人:
KATO, NOBUYUKI,IKEDA, MASANORI
申请号:
EP09811491
公开号:
EP2319916A4
申请日:
2009.09.01
申请国别(地区):
EP
年份:
2012
代理人:
摘要:
According to the present invention, an HCV replicon-replicating cell is produced by a production method including a step of introducing RNA containing an HCV replicon sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. Further, a full-length HCV RNA-replicating cell is produced by a production method including a step of introducing RNA containing a full-length HCV genome sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. The use of these cells enables the construction of an HCV life cycle reproduction system that is derived from a cell line other than the HuH-7 cell line and that has capabilities equivalent to those of an HCV life cycle reproduction system derived from the HuH-7 cell line.